MicroBiology

RNAi Analysis refers to RNA interference analysis. It is the study as well as utilization of the small interfering RNAs that play a vital role in silencing the expression of genes, in the order of one gene at a time. The RNAi Analysis is very useful in describing the network of the gene expression pathways. It also aids in validation of the drug target and thus, results in the invention of new medicines.

The RNAi Analysis is functional to the genomic studies, as well. Researchers have now began applying the RNAi for scanning all the genomes. This helps in determining the network of phenotypic functions as well as the developmental pathways. The technology of the RNAi is a straightforward technology, on which you can rely completely for accurate and precise results. The primary aim of this technique is to selectively silence a particular target mRNAs.

RNAi analysis is sometimes also termed as Reverse Transfection. Reverse transfection is a process wherein the mammalian cells are transfected with the help of the spotted arrays of the DNA plasmid. Thus, this permits the analysis of transfections in parallel, in large numbers. The vectors to be expressed are displayed with a matrix containing the lipid transfection reagents on a microarray plate, slide or dish with the Genetix Array System. This array is later on developed in the adherent cell culture in such a way that the monolayer of the cells grows over the surface of the slide and the cell patches are transfected. The genes to be transfected are frequently cloned as fusions to the fluorescent reporter proteins. This can be used to observe the expression of genes after the transfection process is complete.

One of the applications where the reverse transfection process is specifically well adapted to the microarray formatting is the RNAi (RNA Interface). Here, the transcription of a particular gene is silenced by the reaction of the siRNA (short interfering RNA) molecules. You can measure the efficacy of the RNAi construction by scanning the slide through the confocal scanner along with the blue laser.

The RNA interface is considered as one of the best methods for effectively knocking down the expression of genes in order to study the functioning of proteins in all the types of cells precisely. The traditional methods for knocking down the genes in a mammalian cell comprises of using the synthetic RNA duplexes. This contains two unmodified twenty-one meroligonucleotides linked together for forming either the small interfering RNAs or short interfering RNAs (shRNA). Nowadays, these traditional duplexes are improved with the use of proprietary chemical alterations for ensuring better RNA interface results.

The RNAi analysis technology is used for functionally assess as well as identify number of genes present in the genome that participates actively in diseased phenotypes. Additionally, this technique offers you effective ways to block the expression of a particular gene and then evaluate its reaction to the chemical compounds or any alterations in the signal pathways. The RNAi is the significant technological advance in the modern age that enables one to visualize directly the effects of loss of functioning of a particular gene in the mammalian cell.

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