Microbial ID

Posted by bhavin | Posted in , , | Posted on 10:49 AM

Microbial ID refers to microbial identification. It meets all of the identification needs to recognize the yeast, bacteria and fungi with the help of the necessary tools. More than 2500 species can be identified with Microbial ID. It provides polyphasic investigation exclusively of the fatty acids and the DNA results. These are combined in, as a single report that enables for higher confirmation as well as clarification if any required, of the ID’s.

The Microbial ID process utilizes carbon source metabolic fingerprints. This helps in analyzing the microbial communities, identifying about more than 1900 species of yeast, bacteria and fungi, and characterizing the microbes. The types of samples tested in Microbial ID include environmental, clinical, pharmaceutical, personal, water and food. The types of tests performed on the samples to be tested are bacillus, E.coli, Shigella, streptococcus, clostridia, listeria, enterobacter, vibrio, campylobacter and yeast and mold.

The best genotypic technique for Microbial ID is the comparative DNA sequencing analysis. The commonly used approach is the sequencing and amplification of all the 500 bp portion of the 16S rRNA gene. A comparison is made between the sequence database and the sequenced information. This contains only the validated sequences of the microbes, ideally.

Microbial Identification is widely used in areas like clinical diagnosis, plant pathology, bioremediation, animal science, dental research, medical microbiology, epidemiology, biopharmaceutical, biodefense, microbial forensics, marine science, entomology, soil science and taxonomy studies.

The most widely used system for Microbial ID is the Sherlock Microbial Identification system. This system is used throughout the world in environmental as well as clinical labs for identifying the anaerobic bacteria, aerobic bacteria and the species of yeast. The Sherlock technique is carried out on a gas chromatographic analysis of the cellular fatty acids methyl ester, also known as FAME. This method enables the entire identification process in a time period of about fifteen minutes from the pure culture.

The Sherlock Microbial ID process can classify up to six bacterial agents. There is additional add-on software known as Sherlock DNA along with the Sherlock Microbial ID system. This additional software can recognize about 2500 species of microbes via 28S and 16S ribosomal RNA gene sequencing. The results of the DNA sequencing when combined with the FAME results can identify 2750 species of microbes. There is an extra feature included in this software that enables the user to compare the samples of HPLC method visually to a reference chromatogram.

Key features of the Microbial ID systems:

The Microbial ID system is in use form a very long time from the year 1985and it is one of the widely accepted techniques to identify the aerobic bacteria. There are widespread libraries of the clinical and the environmental organisms. The identification of the different species can be completed in only fifteen minutes. This automated naming and analysis system is very easy to use. It does not require any biochemical cards, gram stains, or upfront tests. It is an extensively comprehensive tool for data analysis and strain tracking. The process of microbial identification is quite reliable.

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