DNA sequencing and methods used for DNA sequencing
Posted by bhavin | Posted in DNA | Posted on 9:47 PM
DNA sequencing
DNA sequencing comprises of the biochemical method which helps to determine the bases of nucleotide, guanine, thymine, adenine, cytosine in the oligonnucleotide of DNA. The elements of DNA sequencing include the heritable genetic information in the mitochondria, plasmids, chloroplasts and nuclei which are the part of developmental programs in all of the living organisms. In the studying the fundamentals of the biological process is it very essential and useful in determine the DNA sequencing. The biological discovery and research are boosted after the advent of DNA sequencing. With the recent technological development DNA sequencing of many animals has also been generated successfully.
Some latest methods used for DNA sequencing are as follows
Number of DNA sequencing are coming up due to large amount of demand for sequencing through low cost. Many private and public organizations and companies are coming up and raising funds for DNA sequencing methods. Many methods are put into implementation these days which have accelerated the pace of sequencing.
Vtro clonal amplification- For the sequencing of the single molecule the methods for the molecular detection are not sufficient, so in number of cases the use of vitro is made for generation of multiple copies of the individual molecule. For the isolation of the individual DNA molecule along with the primer-coated beads Emulation PCR is used often. A chain reaction then coats the head with the help of the clonal copies of isolated library molecule, once this is done then immobilization of heads takes place and then they are sequenced.
Parallelized sequencing- When the DNA sequences get physically localized on the surface number of approaches can be adopted which can determine the DNA sequences of all the locations in parallel. By the use of DNA polymerase for the process of DNA synthesis for the identification of the bases can be done for the Sequencing by synthesis.
There are number of other methods which can be implemented for DNA sequencing using parallel ways.
DNA sequencing comprises of the biochemical method which helps to determine the bases of nucleotide, guanine, thymine, adenine, cytosine in the oligonnucleotide of DNA. The elements of DNA sequencing include the heritable genetic information in the mitochondria, plasmids, chloroplasts and nuclei which are the part of developmental programs in all of the living organisms. In the studying the fundamentals of the biological process is it very essential and useful in determine the DNA sequencing. The biological discovery and research are boosted after the advent of DNA sequencing. With the recent technological development DNA sequencing of many animals has also been generated successfully.
Some latest methods used for DNA sequencing are as follows
Number of DNA sequencing are coming up due to large amount of demand for sequencing through low cost. Many private and public organizations and companies are coming up and raising funds for DNA sequencing methods. Many methods are put into implementation these days which have accelerated the pace of sequencing.
Vtro clonal amplification- For the sequencing of the single molecule the methods for the molecular detection are not sufficient, so in number of cases the use of vitro is made for generation of multiple copies of the individual molecule. For the isolation of the individual DNA molecule along with the primer-coated beads Emulation PCR is used often. A chain reaction then coats the head with the help of the clonal copies of isolated library molecule, once this is done then immobilization of heads takes place and then they are sequenced.
Parallelized sequencing- When the DNA sequences get physically localized on the surface number of approaches can be adopted which can determine the DNA sequences of all the locations in parallel. By the use of DNA polymerase for the process of DNA synthesis for the identification of the bases can be done for the Sequencing by synthesis.
There are number of other methods which can be implemented for DNA sequencing using parallel ways.
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